Tool using BRET for determination of CRISPR/Cas9 restriction efficiency

The technology comprises a new method involving bioluminescence resonance energy transfer (BRET) ratio for determination of restriction efficiency of endonucleases or endonuclease systems comprising a guide-RNA that are used for induction of double-strand breaks at a DNA target sequence of interest for genome editing approaches. The method is suitable for high throughput analyses of potential DNA target sites in combination with different endonucleaseguide-RNA complexes, e.g. CRISPR-Cas9-gRNA complexes.


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